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• Introduction

  • Background

    Introduce the importance and existing research status of the monkeypox virus helicase.

  • Research Purpose

    Clarify the objectives and significance of this research.

  • Research Significance

    Highlight the importance and potential impact of studying the monkeypox virus helicase.

  • Research Content

    Outline the main content and scope of the research.

  • Research Ideas

    Describe the overall ideas and framework of the research.

  • Research Methods

    Summarize the methods used in the research and data sources.

  • Structure and Framework of the Paper

    Introduce the arrangement and overview of chapters in this paper.

  • Innovations of the Paper

    Highlight the innovative aspects of the research.

• Overview of Monkeypox Virus Helicase

  • Structure and Function of Monkeypox Virus Helicase

    Explain the structure and function of the monkeypox virus helicase.

  • Evolutionary Analysis of Monkeypox Virus Helicase

    Analyze the evolutionary characteristics of the monkeypox virus helicase.

  • Comparison with Other Viral Helicases

    Compare the monkeypox virus helicase with other viral helicases.

  • Current Research Progress

    Summarize the current research progress on the monkeypox virus helicase.

• Mechanisms of Monkeypox Virus Helicase

  • Replication Mechanism

    Discuss the replication mechanism of the monkeypox virus helicase.

  • Interaction with Host Proteins

    Explore the interaction between the monkeypox virus helicase and host proteins.

  • Structural Basis for Function

    Analyze the structural basis that underlies the function of the monkeypox virus helicase.

  • Regulation Mechanisms

    Discuss the regulation mechanisms of the monkeypox virus helicase.

• Biophysical Properties of Monkeypox Virus Helicase

  • Thermodynamic Properties

    Examine the thermodynamic properties of the monkeypox virus helicase.

  • Kinetic Properties

    Analyze the kinetic properties of the monkeypox virus helicase.

  • Stability Analysis

    Evaluate the stability of the monkeypox virus helicase under different conditions.

  • Interactions with Small Molecules

    Investigate the interactions between the monkeypox virus helicase and small molecules.

• Experimental Techniques for Studying Monkeypox Virus Helicase

  • Expression and Purification

    Describe techniques for expressing and purifying the monkeypox virus helicase.

  • Enzyme Assays

    Outline the enzyme assays used to study the activity of the monkeypox virus helicase.

  • Crystallography and NMR Studies

    Summarize crystallographic and NMR studies of the monkeypox virus helicase.

  • Single-Molecule Techniques

    Discuss single-molecule techniques used to study the monkeypox virus helicase.

• Clinical and Therapeutic Implications

  • Role in Disease Pathogenesis

    Explore the role of the monkeypox virus helicase in disease pathogenesis.

  • Drug Targets

    Identify potential drug targets related to the monkeypox virus helicase.

  • Antiviral Strategies

    Discuss antiviral strategies targeting the monkeypox virus helicase.

  • Future Research Directions

    Propose future research directions for understanding and combating the monkeypox virus helicase.